Sixty simple sequence repeat markers for use in the Festuca-Lolium complex of grasses

So far only very few simple sequence repeat (SSR) markers developed from grass species have had their primer sequences published. To make more markers available to the scientific community, we isolated and sequenced 256 microsatellite-containing clones from four genome libraries of a Lolium multiflorum x Festuca glaucescens F(1) hybrid following enrichment in (TC)(n), (TG)(n), or both repeats. In this work, we report the primer sequences of 60 SSRs including preliminary results of polymorphism for mapping.