期刊
HISTOCHEMISTRY AND CELL BIOLOGY
卷 123, 期 4-5, 页码 335-346出版社
SPRINGER
DOI: 10.1007/s00418-005-0788-z
关键词
kidney; fibroblast; myofibroblast; epithelial mesenchymal transition; thiazide; FSP1; S100A4; renal interstitium
Fibrosis is considered as a central factor in the loss of renal function in chronic kidney diseases. The origin of fibroblasts and myofibroblasts that accumulate in the interstitium of the diseased kidney is still a matter of debate. It has been shown that accumulation of myofibroblasts in inflamed and fibrotic kidneys is associated with upregulation of fibroblast-specific protein 1 (FSP1, S100A4), not only in the renal interstitium but also in the injured renal epithelia. The tubular expression of FSP1 has been taken as evidence of myofibroblast formation by epithelial - mesenchymal transition (EMT). The identity of FSP1/ S100A4 cells has not been defined in detail. We originally intended to use FSP1/ S100A4 as a marker of putative EMT in a model of distal tubular injury. However, since the immunoreactivity of FSP1 did not seem to fit with the distribution and shape of fibroblasts or myofibroblasts, we undertook the characterization of FSP1/ S100A4-expressing cells in the interstitium of rodent kidneys. We performed immunolabeling for FSP1/ S100A4 on thin cryostat sections of perfusion-fixed rat and mouse kidneys with peritubular inflammation, induced by thiazides and glomerulonephritis, respectively, in combination with ecto-5'-nucleotidase ( 5'NT), recognizing local cortical peritubular fibroblasts, with CD45, MHC class II, CD3, CD4 and Thy 1, recognizing mononuclear cells, with alpha smooth muscle actin (alpha SMA), as marker for myofibroblasts, and vimentin for intracellular intermediate. laments in cells of mesenchymal origin. In the healthy interstitium of rodents the rare FSP1/S100A4+ cells consistently co-expressed CD45 or lymphocyte surface molecules. Around the injured distal tubules of rats treated for 3 - 4 days with thiazides, FSP1+/ S100A4+, 5'NT+, alpha SMA+, CD45+ and MHC class II+ cells accumulated. FSP1+/ S100A4+ cells consistently co-expressed CD45. In the inflamed regions, aSMA was co-expressed by 5'NT+ cells. In glomerulonephritic mice, FSP1+/ S100A4+ cells co-expressed Thy 1, CD4 or CD3. Thus, in the inflamed interstitium around distal tubules of rats and of glomerulonephritic mice, the majority of FSP1+ cells express markers of mononuclear cells. Consequently, the usefulness of FSP1/S100A4 as a tool for detection of (myo)fibroblasts in inflamed kidneys and of EMT in vivo is put into question. In the given rat model the consistent coexpression of aSMA and 5' NT suggests that myofibroblasts originate from resident peritubular fibroblasts.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据