期刊
JOURNAL OF PHYSIOLOGY AND BIOCHEMISTRY
卷 61, 期 2, 页码 353-361出版社
SPRINGER
DOI: 10.1007/BF03167052
关键词
diet-induced obesity; PPAR; RAR; RXR; nuclear receptors; adipose tissue
The aim of this study was to determine the effects of cafeteria diet containing control or elevated level of vitamin A on the expression of nuclear receptors in adipose tissue. Male Wistar rats were submitted to 3 experimental diets during 8 weeks, a standard diet and two hyper-energetic, hyperlipidic cafeteria diets containing normal (Caf) or higher (Caf+) vitamin A level. During the experiment, body weights and energy intakes were measured. At the end of the experimental period, subcutaneous adipose tissue (Swat) and all the fat mass were removed and weighted. Nuclear receptors mRNA levels of RAR alpha, RAR gamma, RXR alpha, PPAR gamma were measured in the Swat by a real-time semi-quantitative RT-PCR method. We observed that energy intake of Caf+ and Caf groups was significantly higher than that of the control group. Despite a higher increase of the energy intake in the Caf group compared to the Caf+ group, no significant difference was observed in the body weight gain of the Caf+ compared to the Caf group. The Caf+ and Caf diets led to a significant increase of adipose tissue in cafeteria groups as observed in the Swat depot. The mRNA levels of PPAR gamma and RXR alpha. were significantly increased in the Caf+ group as compared to control group, with a significant positive correlation between these two parameters. Expressions of RARa and RAR gamma were not modified in experimental groups compared to controls. In conclusion, 8-week exposure to cafeteria diets with normal and higher levels of vitamin A led to an increase of adiposity in rats, associated, only in the group fed with the higher vitamin A level cafeteria diet, with an increase of PPAR gamma and RXR alpha expressions in subcutaneous adipose tissue.
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