期刊
JOURNAL OF BACTERIOLOGY
卷 187, 期 11, 页码 3889-3893出版社
AMER SOC MICROBIOLOGY
DOI: 10.1128/JB.187.11.3889-3893.2005
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资金
- FIC NIH HHS [TW00106, TW006045, R03 TW006045] Funding Source: Medline
Citrobacter freundii cells produce L-methionine gamma-lyase when grown on a medium containing L-methionine. The nucleotide sequence of the hybrid plasmid with a C. freundii EcoRI insert of about 3.0 kbp contained two open reading frames, consisting of 1,194 nucleotides and 1,296 nucleotides, respectively. The first one (denoted megL) encoded L-methionine gamma-lyase. The enzyme was overexpressed in Escherichia coli and purified. The second frame encoded a protein belonging to the family of permeases. Regions of high sequence identity with the 3'-terminal part of the C freundii megL gene located in the same regions of Salmonella enterica serovar Typhimurium, Shigella flexneri, E. coli, and Citrobacter rodentium genomes were found.
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