期刊
PLOS BIOLOGY
卷 3, 期 6, 页码 1047-1061出版社
PUBLIC LIBRARY SCIENCE
DOI: 10.1371/journal.pbio.0030150
关键词
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资金
- MRC [MC_U117565642] Funding Source: UKRI
- Medical Research Council [MC_U117565642] Funding Source: researchfish
- Medical Research Council [MC_U117565642] Funding Source: Medline
- NIAID NIH HHS [AI45073, R01 AI045073] Funding Source: Medline
- NIGMS NIH HHS [R01 GM041514, GM41514] Funding Source: Medline
Interactions between B and T cells are essential for most antibody responses, but the dynamics of these interactions are poorly understood. By two-photon microscopy of intact lymph nodes, we show that upon exposure to antigen, B cells migrate with directional preference toward the B-zone-T-zone boundary in a CCR7-dependent manner, through a region that exhibits a CCR7-ligand gradient. Initially the B cells show reduced motility, but after 1 d, motility is increased to approximately 9 mu m/min. Antigen-engaged B cells pair with antigen-specific helper T cells for 10 to more than 60 min, whereas non-antigen-specific interactions last less than 10 min. B cell-T cell conjugates are highly dynamic and migrate extensively, being led by B cells. B cells occasionally contact more than one T cell, whereas T cells are strictly monogamous in their interactions. These findings provide evidence of lymphocyte chemotaxis in vivo, and they begin to define the spatiotemporal cellular dynamics associated with T cell-dependent antibody responses.
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