期刊
BIOMEDICAL CHROMATOGRAPHY
卷 19, 期 5, 页码 350-354出版社
JOHN WILEY & SONS LTD
DOI: 10.1002/bmc.456
关键词
5-aminosalicylic acid; acetyl-5-aminosalicylic acid; human plasma; reversed-phase HPLC; electrochemical detection; method validation
A high-performance liquid chromatographic method was developed, validated and applied to the simultaneous determination of 5-aminosalicylic acid (5-ASA) and its acetylated metabolite (acetyl-5-ASA) in human plasma. The method involves liquid-liquid extraction with methanol followed by isocratic reversed-phase chromatography on a Kromasil KR100 C-18 column with electrochemical detection. The recovery, selectivity, linearity, precision and accuracy of the method were evaluated from spiked human plasma samples. The effects of mobile phase composition, buffer concentration, mobile phase pH and concentration of organic modifiers on retention of 5-ASA, acetyl 5-ASA and internal standard were investigated. Limits' of detection were 5 ng/mL for 5-ASA and 10 ng/mL for acetyl-5-ASA, respectively. The method can be used for supporting therapeutical drug monitoring and pharmacokinetic studies. Copyright (c) 2004 John Wiley & Sons, Ltd.
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