期刊
AMERICAN JOURNAL OF PHYSIOLOGY-REGULATORY INTEGRATIVE AND COMPARATIVE PHYSIOLOGY
卷 288, 期 6, 页码 R1682-R1688出版社
AMER PHYSIOLOGICAL SOC
DOI: 10.1152/ajpregu.00727.2004
关键词
omega-3 polyunsaturated fatty acids; obesity; adipose tissue; diabetes
类别
资金
- NIDDK NIH HHS [DK-35747] Funding Source: Medline
Eicosapentaenoic acids, has been shown to stimulate leptin mRNA expression and secretion in 3T3- L1 cells. However, other studies have reported inhibitory effects of EPA on leptin expression and secretion in vivo and in vitro. To determine the direct effects of EPA on basal and insulin- stimulated leptin secretion, isolated rat adipocytes were incubated with EPA in the absence and presence of insulin. EPA ( 10, 100, and 200 M) increased basal leptin gene expression and secretion (+ 43.8%, P < 0.05; + 71.1%, P < 0.01; and + 73.7%, P < 0.01, respectively). EPA also increased leptin secretion in the presence of 1.6 nM insulin; however, the effect was less pronounced than in the absence of it. Because adipocyte glucose and lipid metabolism are involved in the regulation of leptin production, the metabolic effects of this fatty acid were also examined. EPA ( 200 mu M) increased basal glucose uptake in isolated adipocytes (+ 50%, P < 0.05). Anaerobic metabolism of glucose, as assessed by lactate production and proportion of glucose metabolized to lactate, has been shown to be inversely correlated to leptin secretion and was decreased by EPA in both the absence and presence of insulin. EPA increased basal glucose oxidation as determined by the proportion of C-14- labeled glucose metabolized to CO2. Lipogenesis (C-14- labeled glucose incorporation into triglyceride) was decreased by EPA in the absence of insulin, whereas lipolysis ( glycerol release) was unaffected. The EPA- induced increase of basal leptin secretion was highly correlated with increased glucose utilization ( r = +0.89, P < 0.01) and inversely related to the anaerobic glucose metabolism to lactate. EPA's effect on insulin-stimulated leptin secretion was not related to increased glucose utilization but was inversely correlated with anaerobic glucose metabolism to lactate ( r= -0.84, P < 0.01). Together, the results suggest that EPA, like insulin, stimulates leptin production by increasing the nonanaerobic/ oxidative metabolism of glucose.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据