期刊
JOURNAL OF NEUROPHYSIOLOGY
卷 93, 期 6, 页码 3582-3593出版社
AMER PHYSIOLOGICAL SOC
DOI: 10.1152/jn.01070.2004
关键词
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资金
- NINDS NIH HHS [NS-38937] Funding Source: Medline
The presence of multiple Na(v)1 isotypes within a neuron and the lack of specific blockers hamper identification of the in vivo roles of sodium current ( I-Na) components, especially during embryonic stages. To identify the functional properties of I-Na components in vivo in developing neurons, we took a molecular genetic approach. Embryonic zebrafish Rohon - Beard (RB) mechanosensory neurons express two different sodium channel isotypes: Na-v 1.1 and Na-v 1.6. To examine the properties of Na-v 1.1- and Na-v 1.6-encoded currents in RB cells at different developmental stages, we eliminated the contribution of Na-v 1.6 and Na-v 1.1 channels, respectively, using an antisense morpholino ( MO) approach. MOs were injected into one-cell stage embryos, and RB sodium currents were recorded using patch-clamp techniques in both conventional whole cell mode as well from nucleated patches. Only a subset of RB cells appeared to be affected by the Na-v 1.1MO. Overall, the effect of the Na-v 1.1MO was a small 25% average reduction in current amplitude. Further, Na-v 1.1MO effects were most pronounced in RB cells of younger embryos. In contrast, the effects of the Na-v 1.6 MO were observed in all cells and increased as development proceeded. These results indicated that developmental upregulation of RB I-Na entailed an increase in the number of functional Na-v 1.6 channels. In addition, analysis of voltage-dependent steady-state activation and inactivation parameters revealed that specific functional properties of channels were also developmentally regulated. Finally, analysis of macho mutants indicated that developmental upregulation of I-Na was absent in RB cells. These results indicate that MOs are a useful tool for the molecular dissection and analysis of ion channel function in vivo.
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