期刊
BRAZILIAN ARCHIVES OF BIOLOGY AND TECHNOLOGY
卷 48, 期 -, 页码 85-95出版社
INST TECNOLOGIA PARANA
DOI: 10.1590/S1516-89132005000400011
关键词
animal cell; CHO-K1; microcarrier; growth; attachment; hemolymph
类别
The aim of this work was to evaluate the potential of hemolymph utilization as a culture medium supplement to cultivate the animal cell CHO-K1. For this purpose 1% v/v of hemolymph was added to DMEM medium containing 10% v/v of FBS and 1 or 4.5 g/L of glucose. The culture was grown in spinnerflasks incubated in a 10% v/v CO2 environment, at 37 degrees C, with the Cytodex 1 microcarrier. Comparing the results obtained from the culture with hemolymph against those without hemolymph, a positive influence of the hemolymph was observed, as the experiment with hemolymph presented a 52% higher cell concentration and a higher productivity of up to 40%.
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