期刊
INTERNATIONAL DAIRY JOURNAL
卷 15, 期 6-9, 页码 785-794出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.idairyj.2004.08.027
关键词
smear cheese; T-RFLP microflora composition; population dynamics
Three Tilsit-type smear-ripened cheeses were manufactured: one was treated with an undefined smear starter mix, the other two were treated with a different defined smear starter mix. The composition of the surface microflora over 8 weeks of ripening was analyzed using Terminal Restriction Fragment Length Polymorphism (T-RFLP) analysis. During the whole period of ripening starter strains were found to be present; additionally, strains apparently acquired from the environment were found. Most of the strains reached a maximum level after 2-4 weeks but were not observed after 8 weeks, except for the Corynebacterium species, which remained as the dominant bacterial genus on the surface of the fully ripened cheese. This study constitutes the first and successful application of an ALFexpress (TM) automatic sequence analyzer to T-RFLP analysis. It was found to be an excellent tool for rapid and specific culture-independent assessment of population composition and dynamics of a cheese system. (c) 2005 Elsevier Ltd. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据