期刊
NATURE MEDICINE
卷 11, 期 6, 页码 678-682出版社
NATURE PUBLISHING GROUP
DOI: 10.1038/nm1247
关键词
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资金
- NCI NIH HHS [R24 CA085140, P01 CA080124, R24 CA 85140, R01 CA085140, R01 CA085140-09, P01 CA 80124, F32 CA 103386, F32 CA103386-02, P01 CA080124-08, F32 CA103386] Funding Source: Medline
- NIBIB NIH HHS [R01 EB001961-06, 5R01 EB 001951-06, R01 EB001961] Funding Source: Medline
A solid tumor is an organ composed of cancer and host cells embedded in an extracellular matrix and nourished by blood vessels. A prerequisite to understanding tumor pathophysiology is the ability to distinguish and monitor each component in dynamic studies. Standard fluorophores hamper simultaneous intravital imaging of these components. Here, we used multiphoton microscopy techniques and transgenic mice that expressed green fluorescent protein, and combined them with the use of quantum dot preparations. We show that these fluorescent semiconductor nanocrystals can be customized to concurrently image and differentiate tumor vessels from both the perivascular cells and the matrix. Moreover, we used them to measure the ability of particles of different sizes to access the tumor. Finally, we successfully monitored the recruitment of quantum dot - labeled bone marrow - derived precursor cells to the tumor vasculature. These examples show the versatility of quantum dots for studying tumor pathophysiology and creating avenues for treatment.
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