期刊
CURRENT EYE RESEARCH
卷 30, 期 6, 页码 479-489出版社
TAYLOR & FRANCIS INC
DOI: 10.1080/02713680590959358
关键词
basic fibroblast growth factor; choroidal neovascularization; endostatin; matrix metalloproteinase-2
Purpose: To investigate the potential role of endostatin, an endogenous angiogenesis inhibitor, in the prevention of choroidal angiogenesis-related disorders. Methods: Bovine choroidal endothelial cells (CEC) were cultured and treated with basic fibroblast growth factor ( bFGF) alone or combined with endostatin at concentrations ranging from 0.1 to 10 mu g/ml. The proliferation and migration of CECs were evaluated by using 3,(4,5-dimethylthiazol-2-yl)-2,5diphenyl tetrazolium bromide (MTT) colorimetric assay and modified Boyden chamber assay, respectively. For evaluating expression and secretion of matrix metalloproteinase-2 ( MMP-2), CEC-conditioned media were subjected to zymography and/or Western blot analysis, and the cells were used for semiquantitative reverse transcription polymerase chain reaction (RT-PCR) analysis. Results: Endostatin did not inhibit bFGF-induced or nonstimulated CEC proliferation ( p > 0.05). The bFGF-induced migration was significantly inhibited by endostatin at concentrations of 1 and 10 mu g/ml ( p < 0.05). The bFGF-upregulated expression of mRNA in CECs and the secretion of MMP-2 protein of CECs were both suppressed by endostatin. Conclusions: Inhibitory effect of endostatin on expression and secretion of MMP-2 and cell migration, but not on proliferation of CECs, could respond to its therapeutic action for choroidal neovascularization-dependent disorders.
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