期刊
GENE
卷 352, 期 -, 页码 100-108出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.gene.2005.04.002
关键词
concerted evolution; gene array; Leishmania major; phylogeny; mini exon gene
资金
- NIAID NIH HHS [AI34536] Funding Source: Medline
The spliced leader RNA gene (SL RNA) repeat is present in large multicopy arrays and has been used as a marker for the diversity of kinetoplastid protozoans. lntra-array variation could affect conclusions made using a randomly isolated repeat as a marker. We examined the Leishmania major (Friedlin) and Trypanosoma cruzi (CL Brener) genome projects for SL RNA repeat sequences in order to assess their homogeneity and the possible effects of sequence variation on taxonomic interpretation. Of the dozens of distinct sequence classes examined, no single copy would bias clustering analyses with regard to other closely related species or isolates. Six dimorphic sites within the T cruzi transcribed region were found to be linked and are predicted to yield a heterogeneous SL RNA population. The variation that exists among the repeats paints a picture of the,broad mechanisms of array maintenance and evolution where site-specific mutations in a single repeat may be spread throughout the array and recombined with existing repeats to create new sequence classes, all occurring under selective pressure to maintain or increase the fitness of the cell line in which these events occur. (c) 2005 Elsevier B.V. All rights reserved.
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