期刊
JOURNAL OF CHROMATOGRAPHY A
卷 1077, 期 2, 页码 110-119出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.chroma.2005.04.088
关键词
gradient elution; method transfer; dwell volume; selectivity
资金
- NIGMS NIH HHS [5R01GM054585-09] Funding Source: Medline
Attempts to theoretically address the problems involved in transferring linear gradient elution methods have been somewhat ad hoc due to the simplifying assumptions usually made in conventional gradient elution theory. Until now, all equations based on the k* parameter of linear gradient elution theory used as the basis for predicting the separation selectivity have not explicitly included the effect of the dwell volume (V-D). Using an exact equation for predicting k*, that is, one which fully accounts in an a priori fashion for V-D, we find a set of simple yet exact equations which unequivocally must be satisfied to transfer an optimized linear gradient elution method from one system (column or instrument or both) to another. These relationships absolutely mandate that a change in the instrument dwell volume requires a proportional change in the column volume; in turn, a change in the column volume requires a proportional change in the flow rate and/or gradient time to maintain a constant gradient steepness. Although we are not the first to suggest these guidelines, this work provides a complete theoretical foundation for these exact guidelines for the maintenance of gradient selectivity for the case of transferring a linear gradient elution method between different columns packed with the same particles and/or between different instruments. (C) 2005 Elsevier B.V. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据