期刊
WORLD JOURNAL OF GASTROENTEROLOGY
卷 11, 期 22, 页码 3375-3384出版社
BAISHIDENG PUBL GRP CO LTD
DOI: 10.3748/wjg.v11.i22.3375
关键词
Epithelial cells; Colon; Commensal bacteria; Cytokines; Chemokines
资金
- USA Public Health Service [AI-41562, PO1 RR12211]
- Royal College of Surgeons Edinburgh
- The Rays of Hope Charitable Trust
- Ann Gloag Fellowship
AIM: To determine if primary murine colonic epithelial cells (CEC) respond to commensal bacteria and discriminate between different types of bacteria. METHODS: A novel CEC: bacteria co-culture system was used to compare the ability of the colonic commensal bacteria, Bacteroides ovatus, E. coli (SLF) and Lactobacillus rhamnosus (LGG) to modulate production of different cytokines (n = 15) by primary CEC. Antibody staining and flow cytometry were used to investigate Toll-like receptor (TLR) expression by CEC directly ex vivo and TLR responsiveness was determined by examining the ability of TLR ligands to influence CEC cytokine production. RESULTS: Primary CEC constitutively expressed functional TLR2 and TLR4. Cultured in complete medium alone, CEC secreted IL-6, MCP-1 and IP-10 the levels of which were significantly increased upon addition of the TLR ligands peptidoglycan (PGN) and lipopolysaccharide (LPS). Exposure to the commensal bacteria induced or up-regulated different patterns of cytokine production and secretion. E. coli induced production of MIP-1 alpha/beta and beta defensin3 whereas B. ovatus and L. rhamnosus exclusively induced MCP-1 and MIP-2 alpha expression, respectively. TNF alpha, RANTES and MEC were induced or up-regulated in response to some but not all of the bacteria whereas ENA78 and IP-10 were up-regulated in response to all bacteria. Evidence of bacterial interference and suppression of cytokine production was obtained from mixed bacterial: CEC co-cultures. Probiotic LGG suppressed E. coli-and B. ovatus-induced cytokine mRNA accumulation and protein secretion. CONCLUSION: These observations demonstrate the ability of primary CEC to respond to and discriminate between different strains of commensal bacteria and identify a mechanism by which probiotic bacteria (LGG) may exert anti-inflammatory effects in vivo. (C) 2005 The WJG Press and Elsevier Inc. All rights reserved.
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