期刊
PROSTATE
卷 64, 期 1, 页码 92-100出版社
WILEY
DOI: 10.1002/pros.20219
关键词
prostate cancer; receptor for advanced glycation end products; amphoterin; invasion
BACKGROUND. Advanced glycation end products (AGE) are produced with normal aging. Recently, some reports indicated that the interaction between AGE and the cognate receptor (RAGE) has a role in cancer dependent. METHODS. We investigated RAGE and amphoterin mRNA expression in prostate cancer cell lines (DU145, PC-3, and LNCaP cells), hormone-refractory prostate cancer tissue,, and paired. prostate cancer and normal prostate (including benign prostatic hyper-untreated primary trophy (BPH)) tissues using real-time quantitative PCR. Moreover, to confirm the AGE- RACE interaction in prostate cancer, DU145 cells stimulated with AGE-bovinescrum albumin (AGE-BSA) were examined by in vitro matrigel assay, cell viability assay, MTT assay, reverse transcription-polymerase chain reaction (RT-PCR), and Western blot. RESULTS. DU145 cells, a hormone-independent prostate cancer cell line, showed the highest RAGE mRNA expression. Amphoterin mRNA was expressed in all three cell lines. In prostate tissues, untreated prostate cancer tissue and hormone-refractory prostate cancer tissue Showed higher RAGE and amphoterin mRNA expression than normal prostate tissue. The AGE- RACE interaction induced the invasion and growth in DU145 cells stimulated with AGE-BSA. CONCLUSIONS. The AGE-RAGE interaction is important in prostate cancer development, and inhibition of this interaction has potential as a new molecular target for cancer therapy or prevention. (c) 2005 Wiley-Liss. Inc.
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