期刊
FEBS LETTERS
卷 579, 期 16, 页码 3401-3410出版社
WILEY
DOI: 10.1016/j.febslet.2005.04.083
关键词
transcription; TRF2/TLF; spermatogenesis; mass spectrometry
Male germ cells specifically express paralogues of components of the general transcription apparatus including ALF a paralogue of TFIIA alpha/beta. We show that endogenous ALF is proteolytically cleaved to give alpha- and beta-subunits and we map the proteolytic cleavage site by mass spectrometry. Immunoprecipitations show that ALF alpha- and beta-subunits form a series of homologous and heterologous complexes with somatic TFIIA which is coexpressed in male germ cells. In addition, we show that ALF is coexpressed in late pachytene spermatocytes and in haploid round spermatids with transcription factor TRF2, and that these proteins form stable complexes in testis extracts. Our observations highlight how cleavage of ALF and coexpression with TFIIA and TRF2 increases the combinatorial possibilities for gene regulation at different developmental stages of spermatogenesis. (c) 2005 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.
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