期刊
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
卷 102, 期 26, 页码 9353-9358出版社
NATL ACAD SCIENCES
DOI: 10.1073/pnas.0503965102
关键词
adult stem cells; electrophysiology; in vitro; neurogenesis; subventricular zone
资金
- NHLBI NIH HHS [R01 HL070143, HL70143] Funding Source: Medline
- NICHD NIH HHS [T32 HD043730, T32HD043730] Funding Source: Medline
- NINDS NIH HHS [NS37556, R21 NS046384, R01 NS037556, NS046384] Funding Source: Medline
The modern concept of neurogenesis in the adult brain is predicated on the premise that multipotent glial cells give rise to new neurons throughout life. Although extensive evidence exists indicating that this is the case, the transition from glial to neuronal phenotype remains poorly understood. A unique monolayer cell-culture system was developed to induce, expose, and recapitulate the entire developmental series of events of subventricular zone (SVZ) neurogenesis. We show here, using immunophentoypic, ultrastructural, electrophysiological, and time-lapse analyses, that SVZ-derived glial fibrillary acidic protein(low)/A2B5(+)/nestin(+) candidate founder cells undergo metamorphosis to eventually generate large numbers of fully differentiated interneuron phenotypes. A model of postnatal neurogenesis is considered in light of known embryonic events and reveals a limited developmental potential of SVZ stem/progenitor cells, whereby ancestral cells in both embryonic and postnatal/adult settings give rise to glia and GABAergic interneurons.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据