期刊
ANALYTICA CHIMICA ACTA
卷 542, 期 2, 页码 169-177出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.aca.2005.03.059
关键词
nuclear magnetic resonance spectroscopy (NMR); urine; phenylketonuria (PKU); maple syrup urine disease (MSUD); principal component analysis (PCA); partial least squares discriminant analysis (PLS-DA)
H-1 NMR-based metabonomics was used for the detection and diagnosis of inborn errors of metabolism from urine samples. ID H-1 NMR spectra from 47 normal, 9 phenylketonuric (PKU) newborns and I maple syrup urine disease (MSUD) child were obtained and investigated. Urine H-1 NMR spectra of normal, PKU and MSUD samples exhibited differences concerning the phenylalanine (Phe) and branched-chain amino acids (leucine, valine, isoleucine) resonances, respectively. Principal component analysis (PCA) and partial least squares discriminant analysis (PLS-DA) were applied in order to establish adequate models for discrimination between pathological and normal samples. Normalization of the spectra was based to the total spectral intensity or to creatinine peak. Different data transformation procedures were used. Discrimination of PKU and MSUD samples from normal samples was achieved by the different models produced by PCA and PLS-DA. Comparing the two methods of statistical analysis, PLS-DA was found to lead to a most proper discrimination when all pathological samples were used. while PCA proved suitable to identify every single pathological sample among the physiological ones. Thus, H-1 NMR in urine can be considered as an alternative to blood spots in order to develop a mass-screening method, which does not require sample pre-treatment and avoids any painful procedure for the newborns. (c) 2005 Elsevier B.V. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据