期刊
MICROVASCULAR RESEARCH
卷 70, 期 1-2, 页码 53-64出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.mvr.2005.04.005
关键词
CCR2; CCL2; endothelial cells; transendothelial migration
资金
- NIMH NIH HHS [R0-1-MH54718] Funding Source: Medline
While the expression of chemokine receptors by endothelial cells is now well established, little is known of the function of these receptors at this cellular locale. However, given that chemokines are instrumental in directing leukocytes to specific parenchymal sites, one possibility is that endothelial chemokine receptors play a role in the process of leukocyte extravasation. To test this hypothesis, we investigated the contribution of CCR2, the major cognate receptor for the chemokine CCL2 (formerly known as MCP-1), to CCL2-stimulated transendothelial migration of macrophages (m) across cultured brain microvascular endothelial cells (BMEC). Specifically, we prepared both BMEC and mO from wild-type (WT) trice and mice deficient in CCR2; i.e., CCR2 (-/-), and compared the ability of WT and CCR2 (-/-) BMEC to support CCL2-stimulated transendothelial migration of WT and CCR2 (-/-) mO. In response to CCL2, WT mO, but not CCR2 (-/-) mO, were Stimulated to migrate across WT BMEC, consistent with the recognized obligatory role for CCR2 ill mediating CCU-stimulated responses. Remarkably, however, neither WT nor CCR2 (-/-) mO were stimulated by CCL2 to migrate across CCR2 (-/-) BMEC. In contrast, both types of mO were able to migrate similarly across both types of BMEC in response to another chemokine-CCL3 (formerly known as MIP-1 alpha)-which utilizes receptors other than CCR2. Lastly, CCL2-induced mO transendothelial migration was blocked by treatment of WT BMEC with pertussis toxin, suggesting that CCR2 is functionally coupled to the inhibitory G protein G alpha(i), Much as it is in other cell types. These results highlight a heretofore-unrecognized role for endothelial CCR2 in mediating transendothelial migration. (c) 2005 Elsevier Inc. All rights reserved.
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