期刊
DEVELOPMENTAL DYNAMICS
卷 233, 期 3, 页码 1140-1148出版社
WILEY
DOI: 10.1002/dvdy.20425
关键词
mouse embryo day 7; light microscopic immunostaining; in situ RT-PCR; laminins; nidogen-1 and nidogen-2; collagen type IV; perlecan
Basement membranes (BM) are specialized structures of the extracellular matrix known to be involved in various early developmental processes. Despite numerous investigations on the localization of BM components, it remains unknown which molecules are expressed in early developmental stages and by which germ layers these proteins are produced. Therefore, we tested for all known laminin chains, nidogens, collagen type IV, and perlecan by means of light microscopic immunostaining and performed in situ reverse transcriptase-polymerase chain reaction to detect the mRNAs specific for laminin alpha 1, laminin beta 1, the alpha 1 chain of collagen type TV, nidogen-2, and perlecan in the early mouse embryo, day 7, in vivo. Only the laminin chains alpha 1, beta 1, and gamma 1 were detected immunohistochemically throughout the entire endodermal and ectodermal BM zones of the embryo proper. The mRNA of laminin alpha 1, laminin beta 1, collagen type IV, nidogen-2 and perlecan were expressed in the ectoderm-derived mesoderm, in the endoderm. as well as in the ectoderm. In contrast, Reichert's membrane was positive for all laminin chains except for the alpha 4, alpha 5, beta 3, and gamma 3 chains. Moreover, maternal epithelial as well as mesenchymal cells expressed laminins, nidogen-1 and nidogen-2, collagen type IV, and perlecan. In conclusion, laminin-1 might be the only laminin isoform in the early mouse embryo that, together with the other main BM components, nidogens, collagen type IV, and perlecan, is synthesized by all three germ layers. (c) 2005 Wiley-Liss, Inc.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据