期刊
PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY
卷 82, 期 3, 页码 205-219出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.pestbp.2004.11.009
关键词
chloroacetanilides; diphenyl ether; glutathione transferase; homoglutathione; herbicide; safener; sulfonylurea
Glutathione transferases in soybean (Gi-nGSTs) involved in herbicide detoxification in cell suspension cultures were purified by S-hexylglutathione affinity chromatography and resolved by a combination of HPLC and SDS-PAGE into 11 polypeptides. Analysis by Western blotting using antisera raised to three previously characterised tau (GmGSTU) class subunits demonstrated that five polypeptides were related to GinGSTU1, three to GmGSTU2, and one to Gin GSTU3. Plants contained a simpler profile of polypeptides, with a single GmGSTU2-like polypeptide predominating. With respect to herbicide detoxification, two GinGSTU2-related polypeptides dominated the activity toward the chloroacetanilide acetochlor, while an unclassified subunit was uniquely associated with the detoxification of diphenyl ethers (acifluorfen.. fornesafen). The inducibility of the different GST subunits was determined in soybean plants exposed to photobleaching diphenyl ethers and the safeners naphtbalic anhydride and dichlormid. GinGSTU3, a GmGSTU1-like polypeptide.. and thiol (homoglutathione) content were induced by all chemical treatments, while two uncharacterised subunits were only induced in plants showing photobleaching. (c) 2005 Elsevier Inc. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据