期刊
JOURNAL OF PLANT PHYSIOLOGY
卷 162, 期 7, 页码 818-822出版社
ELSEVIER GMBH
DOI: 10.1016/j.jplph.2005.04.021
关键词
antioxidants; flavonoids; glutathione; ochratoxin A; oxidative stress; alpha-tocopherol
It has been shown that oxidative damage contributes to the wide range of toxic effects of the mycotoxin ochratoxin A (OTA). Therefore, we examined the effects of alpha-tocopherol (alpha-TOC) and different polyphenols - catechin (CAT), daidzein (DAI), epicatechin (EC), epigallocatechin gallate (EGCG), genistein (GEN), and quercetin (QUE) - on OTA-induced cytotoxicity in HepG2 liver cells. Incubation of HepG2 cells with increasing concentrations of OTA resulted in a dose- and time-dependent cytotoxicity as measured by the neutral red assay. Half lethal concentrations (LC50) Of OTA were 35 and 10 mu M after 48 and 72 h incubation, respectively. Incubation of HepG2 cells with alpha-TOC as well as with different polyphenols (exhibiting different antioxidant potency as determined by the FRAP, TEAC and DPPH assays) did not counteract OTA-induced cytotoxicity. These findings indicate that OTA may exert its toxic effects by affecting other hepatic mechanisms than those directly modulated by alpha-TOC and polyphenols. (c) 2005 Elsevier GmbH. All rights reserved.
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