期刊
JOURNAL OF VIROLOGICAL METHODS
卷 127, 期 1, 页码 80-86出版社
ELSEVIER
DOI: 10.1016/j.jviromet.2005.02.016
关键词
unrecognized viral agents; mRNA; cDNA; subtraction hybridization; long-PCR; nested-PCR; mung bean nuclease; cDNA library
For epidemic control, rapid identification and characterization of the responsible unknown agent are crucial. To address this critical question, a method was developed for virus discovery based on a flexible nested-PCR subtraction hybridization. As a positive control, we used hepatitis C virus as a hypothetical unrecognized virus and discover it in the sample. Using template-switching universal long-PCR to produce large quantities of cDNA, our nested-PCR-based subtractive hybridization coupled with a single-strand deletion technology removed most of the common cDNA. Following subtraction hybridization, a cDNA library was constructed and displayed by differential reverse dot blot hybridization. This new genomic subtraction hybridization method will be ideally suited to identify rapidly any previously unrecognized viral agent. Published by Elsevier B.V
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据