Cytokines locally produced by lymphocytes removed from the hypertrophic nasopharyngeal and palatine tonsils

Objective: Human palatine tonsils and the nasopharyngheal. tonsil are the largest components of the Waldeyer's ring. Subepithetial and intraepithetiat lymphocytes of human adenoids and tonsils are responsible for the local and the systemic immune response. We studied the cytokine production by Lymphoid cells isolated from 16 nasopharyngeal. tonsils (adenoid) and 9 palatine tonsils surgically removed by from 25 children (aged from 4 to 15 years) suffering from tonsil hypertrophy. Methods: We evaluated (by the cytometry method, using BD Bioscience kits, San Diego, CA) the concentration of IL-2, IL-4, IL-5, IL-10, TNF alpha and IFN gamma released from human peripheral blood mononuclear cells (MC) (activated or not activated by phytohaemagglutinin (PHA)) cultured in vitro during 72 h. The fluorescence-activated cell sorter (FACS) analysis was also performed and the percentage of mononuclear cells (unstimulated or activated by phorbol acetate during 24 h) stained with the monoclonal antibodies anti-CD3 containing the intracellular cytokines was calculated. Results: The increased secretion of IL-2, IL-4, IL-5, TNF alpha and IFN gamma from PHA activated palatine origin immune cell cultures, as compared to adenoids, was revealed. The higher mobilization (Delta%) of CD3(+) T-lymphocytes containing IL-12 in palatine cell cultures (798.5 +/- 276.29%), in comparison with to the adenoids (298.5 +/- 49.16%; p <= 0.05), was also noted. Conclusion: In palatine tonsils, as compared to adenoids, the cellular immune (Th1) response dominates over humoral. immune (Th2) reaction. (c) 2005 Etsevier Ireland Ltd. All rights reserved.