期刊
APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
卷 175, 期 2, 页码 974-987出版社
SPRINGER
DOI: 10.1007/s12010-014-1341-0
关键词
beta-LG; Oxaliplatin; FRET; Fluorescence; Circular dichroism; Molecular docking
The potential carrier role of beta-lactoglobulin (beta-LG) and its interactions with oxaliplatin were studied using various spectroscopic techniques (fluorescence, UV-visible, and circular dichroism (CD)) in an aqueous medium at two temperatures of 25 and 37 A degrees C in combination with a molecular docking study. Fluorescence measurements have shown that the observed quenching is a combination of static and dynamic quenching with a predominant contribution of static mode. The presence of a single binding site located in the internal cavity of the beta-barrel of beta-LG was confirmed by molecular docking calculations. Thermodynamic data as well as molecular docking indicated that the hydrophobic interactions dominate in the binding site. Results of fluorescence resonance energy transfer (FRET) measurements in combination with docking results imply that resonance energy transfer occurs between beta-LG and its ligand oxaliplatin. Additionally, CD results revealed that oxaliplatin binding has no influence on the beta-LG structure. The molecular docking results indicate that docking may be an appropriate method for the prediction and confirmation of experimental results. Complementary molecular docking results may be useful for the determination of the binding mechanism of proteins such as beta-LG in pharmaceutical and biophysical studies providing new insight in the novel pharmacology and new solutions in the formulation of advanced oral drug delivery systems.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据