期刊
APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
卷 174, 期 2, 页码 708-718出版社
SPRINGER
DOI: 10.1007/s12010-014-1101-1
关键词
Solid-state cultivation; Drechslera (Helminthosporium) monoceras; Oxygen transfer; K(L)a; Packed bed bioreactor; Wheat bran
资金
- CNPq
- CAPES
The aim of this work was to study oxygen transfer as a function of the initial moisture content in solid-state cultivation under controlled moisture conditions. The use of controlled moisture conditions prevents drastic changes in the medium during cultivation, allowing the use of a pseudo-steady-state model to estimate the overall oxygen mass transfer coefficient (K (L) a) in the biofilm around the solid particles. Drechslera (Helminthosporium) monoceras, an aerobic mold that produces allergenic proteins, was cultured on wheat bran in a packed bed column bioreactor. The bed height (30 mm) and air flow rate (0.4 L/min) were selected to implement moisture control. The results show that there is an optimal moisture content (35 %) at which a lower biofilm thickness and packing of the bed improves K (L) a. However, a higher biomass growth was obtained at 45 % moisture. The different patterns of biomass growth demonstrate the importance of the balance between aerial and film growth in solid-state cultivation. These results contribute to the understanding of oxygen transfer in solid fermentation, optimization of processes, and production of allergen extracts from D. (Helminthosporium) monoceras biomass.
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