期刊
APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
卷 172, 期 8, 页码 3902-3912出版社
SPRINGER
DOI: 10.1007/s12010-014-0819-0
关键词
Inflammation; Inflammasome; Caspase-1; NALP3; ASC
资金
- Basic Science Research Program through the National Research Foundation of Korea (NRF) of the Ministry of Education, Science and Technology [2013009083]
- Korea Healthcare Technology R&D project, Ministry of Health & Welfare, Republic of Korea [HI13C1449]
NALP3 inflammasome, which is an inflammatory caspase-activating complex, is composed of three proteins: NALP3 (an NOD-like receptor), an apoptosis-associated speck-like protein containing a caspase recruitment domain (ASC), and caspase-1. NALP3 senses danger signals, while ASC is an adaptor molecule containing two protein interaction modules: pyrin domain (PYD) and caspase recruitment domain (CARD). Caspase-1 is a cysteine protease that uses cysteine as a nucleophile and has a CARD domain for protein interaction. During inflammasome formation, the ASC adaptor acts as a bridge between caspase and NOD-like receptor (NLR) by offering the CARD for CARD-CARD interactions and PYD for PYD-PYD interactions. In the current study, we successfully purified and characterized NALP3 PYD and ASC PYD. The results showed that ASC PYD easily self-oligomerized under physiological conditions, and this self-oligomerization of the ASC PYD prevented complex formation with NALP3 PYD in vitro.
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