期刊
SCIENCE
卷 309, 期 5731, 页码 125-127出版社
AMER ASSOC ADVANCEMENT SCIENCE
DOI: 10.1126/science.1112440
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资金
- NIGMS NIH HHS [GM23441] Funding Source: Medline
Myxococcus xanthus cells can glide forward by retracting type IV pili. Tgl, an outer membrane lipoprotein, is necessary to assemble pili. Tgl(+) mutants can be transiently stimulated if brought into end-to-end contact with tgl(+) donor cells. By separating the stimulated recipient cells from donor cells, we found that Tgl protein was transferred from the donors to the rescued recipient cells. Mutants lacking CglB lipoprotein, which is part of a second gliding engine, could also be stimulated, and CglB protein was transferred from donor to recipient cells. The high transfer efficiency of Tgl and CglB proteins suggests that donor and recipient cells briefly fuse their outer membranes.
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