期刊
APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
卷 172, 期 4, 页码 2197-2204出版社
HUMANA PRESS INC
DOI: 10.1007/s12010-013-0651-y
关键词
Lipomyces starkeyi; Microbial oil; Corncob acid hydrolysate; Biodiesel
资金
- project of Guangzhou Science and Technology [2013J4300031]
- Support Plan Project of the National Science and Technology [2012BAD32B07]
- National Natural Science Foundation of China [51378486, U1261116]
- Natural Science Foundation of Guangdong Province [S2012040007546]
- Foundation of Director of Guangzhou Institute of Energy Conversion, Chinese Academy of Sciences [y107rf1001]
For the first time, corncob acid hydrolysate was used for microbial oil production by the oleaginous yeast Lipomyces starkeyi. After hydrolysis by dilute sulfuric acid, corncob could turn into an acid hydrolysate with a sugar concentration of about 42.3 g/L. Detoxified by overliming and absorption with activated carbon, the corncob hydrolysate could be used by L. starkeyi efficiently that a total biomass of 17.2 g/L with a lipid content of 47.0 % (corresponding to a lipid yield of 8.1 g/L) and a lipid coefficient of 20.9 could be obtained after cultivation on the corncob hydrolysate for 8 days. Therefore, L. starkeyi is a promising strain for microbial oil production from lignocellulosic biomass. Glucose and xylose were used by L. starkeyi simultaneously during lipid fermentation while arabinose could not be utilized by it. Besides, the lipid composition of L. starkeyi was similar to that of vegetable oils; thus, it is a promising feedstock for biodiesel production.
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