期刊
APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
卷 169, 期 5, 页码 1531-1545出版社
SPRINGER
DOI: 10.1007/s12010-013-0094-5
关键词
Bioethanol; Jerusalem artichoke; Stalk; Kluyveromyces marxianus; Simultaneous saccharification and fermentation
资金
- Technology Development Program for Agriculture and Forestry, Ministry for Food, Agriculture, Forestry and Fisheries, Republic of Korea
- New & Renewable Energy Technology Development Program of the Korea Institute of Energy Technology Evaluation and Panning (KETEP) [2011T100200066]
- Korea government Ministry of Knowledge Economy
Jerusalem artichoke is a low-requirement sugar crop containing cellulose and hemicellulose in the stalk and a high content of inulin in the tuber. However, the lignocellulosic component in Jerusalem artichoke stalk reduces the fermentability of the whole plant for efficient bioethanol production. In this study, Jerusalem artichoke stalk was pretreated sequentially with dilute acid and alkali, and then hydrolyzed enzymatically. During enzymatic hydrolysis, approximately 88 % of the glucan and xylan were converted to glucose and xylose, respectively. Batch and fed-batch simultaneous saccharification and fermentation of both pretreated stalk and tuber by Kluyveromyces marxianus CBS1555 were effectively performed, yielding 29.1 and 70.2 g/L ethanol, respectively. In fed-batch fermentation, ethanol productivity was 0.255 g ethanol per gram of dry Jerusalem artichoke biomass, or 0.361 g ethanol per gram of glucose, with a 0.924 g/L/h ethanol productivity. These results show that combining the tuber and the stalk hydrolysate is a useful strategy for whole biomass utilization in effective bioethanol fermentation from Jerusalem artichoke.
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