期刊
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
卷 382, 期 5, 页码 1288-1293出版社
SPRINGER HEIDELBERG
DOI: 10.1007/s00216-005-3254-9
关键词
food analysis; mycotoxins; ochratoxin A; rice; HPLC; fluorescence detection
A sensitive and accurate analytical method for the determination of ochratoxin A (OTA) in rice, based on extraction with phosphate-buffered saline/methanol, an immunoaffinity column (IAC) for clean-up, and high performance liquid chromatography with fluorescence detection (HPLC-FD), is described. The limit of quantification of the proposed method was 0.05 mu g kg(-1). Recovery of OTA from rice samples spiked at 0.05 mu g kg(-1) was 92%, with a within-day RSD of 5.4%. The proposed method was applied to 42 rice samples from Portugal and the presence of OTA was found in six samples at concentrations ranging from 0.09 to 3.52 mu g kg(-1). The identification of OTA was confirmed by methyl ester derivatization and then HPLC analysis. The daily intake of OTA by the Portuguese population was also estimated.
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