期刊
APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
卷 170, 期 4, 页码 951-961出版社
SPRINGER
DOI: 10.1007/s12010-013-0234-y
关键词
Phenol hydroxylase; Chloro-substituted indoles; Biotransformation; Response surface methodology
资金
- National Creative Research Group from the National Natural Science Foundation of China [51121062]
- National Natural Science Foundation of China [51108120, 51078054, 51178139]
- 4th Special Financial Grant from the China Postdoctoral Science Foundation [201104430]
Recombinant Escherichia coli cells expressing phenol hydroxylase (designated as strain PHIND) were used to synthesize chloro-substituted indigoids by the transformation of indoles. The optimal conditions for the biotransformation of 4- and 7-chloroindole were determined by response surface methodology. Biotransformation kinetic assays revealed that strain PHIND showed high catalytic efficiency for 4- and 7-chloroindole. The formation rate of 7,7'-dichloroindigo (1.35 unit/mg cell dry weight) by strain PHIND was 1.14-fold higher than that of 4,4'-dichloroindigo. The intermediates of 7-chloroindole biotransformation were identified by high-performance liquid chromatography-mass spectroscopy, and the biotransformation mechanism was also proposed. These results suggested that there was a potential application of strain PHIND in the biotransformation of chloro-substituted indoles to valuable indigoids.
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