期刊
APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
卷 172, 期 4, 页码 1859-1865出版社
SPRINGER
DOI: 10.1007/s12010-013-0625-0
关键词
Chemiluminescent probearray; Co3O4-SiO2 mesoporous nanocomposite material; Immobilization; Lactose
资金
- Fundamental Research Funds for the Central Universities [GK 20091004]
- National Natural Science Foundation of China [30872371]
This work presents a new chemiluminescent (CL) probe array assay. The new type CL probe array is based on enzyme mimics of Co3O4-SiO2 mesoporous nanocomposite material, which not only have an excellent catalytic effect on the luminol-H2O2 CL reaction in an alkaline medium but also can be used for the immobilization of enzymes. The linear range of the lactose concentration is 3.0x10(-7) to 1.0x10(-5) g mL(-1) and the detection limit is 6.9x10(-8) g mL(-1). beta-Galactosidase and glucose oxidase were selected as a model for enzyme assays to demonstrate the applicability of Co3O4-SiO2 mesoporous nanocomposite material in multienzyme immobilization. The novel bifunctional CL probe array has been successfully applied to the determination of lactose in milk.
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