期刊
APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
卷 169, 期 1, 页码 290-300出版社
SPRINGER
DOI: 10.1007/s12010-012-9986-z
关键词
Spectral studies; Lysozyme; Structural changes; Activity; Ionic liquids
资金
- National Natural Science Foundation of China [20806053]
- China Postdoctoral Science Foundation [200902274]
Lysozyme crystals in the presence of 1-butyl-3-methylimidazolium tetrafluoroborate ([C(4)mim]BF4), 1-butyl-3-methylimidazolium chloride ([C(4)mim]Cl), 1-butyl-3-methylimidazolium bromide([C(4)mim]Br), and 1,3-dimethylimidazolium iodine([dmim]I) were prepared, and the influence of ionic liquids (ILs) on the structure and activity change of lysozyme was investigated. Fourier transform infrared spectroscopy revealed the major secondary structures of alpha-helix and beta-sheet for lysozyme. It was interesting to note that increases of the band near 2,935 and 1,656 cm(-1) from Raman spectroscopy are attributed to the unfolding of lysozyme molecules. A shift in amide III from 1,230 to 1,270 cm(-1) in adding [dmim]I occurs, indicating a transformation from beta-sheet to random coil. With regard to adding [C(4)mim]BF4, [C(4)mim]Cl, and [C(4)mim]Br, alpha-helix and beta-sheet are the predominant structures for lysozyme. The activity study showed that the ILs used brought a positive effect. Especially, [dmim]I leads to a drastic increase in relative activity, and its value reaches 50 %.
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