期刊
APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
卷 168, 期 5, 页码 1197-1211出版社
HUMANA PRESS INC
DOI: 10.1007/s12010-012-9850-1
关键词
beta-Galactosidase; Entrapment; Galacto-oligosaccharides; Whey; Lactose; Continuous operation
资金
- Fundacao para a Ciencia e a Tecnologia (Portugal)
The synthesis of galacto-oligosaccharides (GOS) by beta-galactosidase immobilized in both polyvinyl alcohol (PVA) lenses and sol-gel carriers was studied and compared with the performance of the free enzyme. PVA-immobilized beta-galactosidase retained 95 % of the initial activity after seven repeated uses and retained 51 % of the initial activity after 3 months of storage, while sol-gel-immobilized beta-galactosidase only retained 39 % of the initial activity under storage. Lactose conversion takes place at a higher rate in the PVA-immobilized beta-galactosidase, while the lowest rate of lactose conversion was noticed with immobilized beta-galactosidase in sol-gel. Continuous production of GOS from either lactose or whey, with PVA-immobilized beta-galactosidase, was performed in a packed-bed reactor. A maximum GOS production of 30 % of total sugars was attained for a 40-% lactose feed solution with a feed rate of 10.8 ml/h, at pH 4.5 and 40 A degrees C, corresponding to a productivity of 117 g/l h. The maximum GOS productivity of 344 g/l h was obtained at a flow rate of 28.7 ml/h. 3-OS and 4-OS were the major types of GOS formed. Conversion of whey in continuous mode resulted in GOS production of 15 % of total sugars and formation of 45 % 3-OS, 40 % 4-OS, and 15 % 5-OS.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据