期刊
BIORESOURCE TECHNOLOGY
卷 96, 期 11, 页码 1304-1310出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.biortech.2004.10.009
关键词
acetate; N and P status; Nostoc muscorum; pH; poly-p-hydroxybutyrate
Accumulation of poly-beta-hydroxybutyrate (PHB) in Nostoc muscorum was studied. Cells harvested at stationary phase of growth depicted maximum accumulation i.e. 8.6% (w/w) of dry cells as compared to lag (4.1%) or logarithmic (6.1%) phases of cultures. In contrast to alkaline pH, acidic pH, continuous illumination and cells grown in presence of combined nitrogen sources, such as NH4Cl and KNO3, were found to affect PHB accumulation negatively. However, P-deficiency and addition of exogenous carbon sources (acetate, glucose, maltose, fructose and ethanol) were found stimulatory for PHB accumulation. In this report PHB accumulation in N. muscorum was boosted up to 35% (w/w) of dry cells when cells supplemented with 0.2% acetate were subjected to dark incubation for 7 days. Further studies are needed at metabolic engineering level or to apply genetic engineering techniques to improve the expression level of PHB photoproduction in cyanobacteria. (c) 2004 Elsevier Ltd. All rights reserved.
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