Enzymatic Hydrolysis of Polylactic Acid Fiber

This study investigated the optimization of the enzymatic processing conditions for polylactic acid (PLA) fibers using enzymes consisting of lipases originating from different sources. The hydrolytic activity was evaluated taking into consideration the pH, temperature, enzyme concentration, and treatment time. The structural change of the PLA fibers was measured in the optimal treatment conditions. PLA fiber hydrolysis by lipases was maximized for lipase from Aspergillus niger at 40 A degrees C for 60 min at pH 7.5 with 60% (owf) concentration, for lipase from Candida cylindracea at 40 A degrees C for 120 min at pH 8.0 with 70% (owf) concentration, and for lipase from Candida rugosa at 45 A degrees C for 120 min at pH 8.0 with 70% (owf) concentration. There was a change in protein absorbance of the treatment solution before and after all lipase treatments. The analyses of the chemical structure change and structural properties of the PLA due to lipase treatment was confirmed by tensile strength, differential scanning calorimetry, wide-angle X-ray scattering diffractometry, Fourier transform infrared spectroscopy, and scanning electron microscopy.