期刊
APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
卷 161, 期 1-8, 页码 67-74出版社
SPRINGER
DOI: 10.1007/s12010-010-8932-1
关键词
Dilute ammonia pretreatment; Sorghum; Ethanol; Lignocellulosic; Biomass; Hydrolysis
资金
- U.S. Department of Energy [DE-FG36-08GO88151]
A new pretreatment technology using dilute ammonium hydroxide was evaluated for ethanol production on sorghum. Sorghum fibers, ammonia, and water at a ratio of 1:0.14:8 were heated to 160 A degrees C and held for 1 h under 140-160 psi pressure. Approximately, 44% lignin and 35% hemicellulose were removed during the process. Hydrolysis of untreated and dilute ammonia pretreated fibers was carried out at 10% dry solids at an enzyme concentration of 60 FPU Spezyme CP and 64 CBU Novozyme 188/g glucan. Cellulose digestibility was higher (84%) for ammonia pretreated sorghum as compared to untreated sorghum (38%). Fermentations with Saccharomyces cerevisiae D(5)A resulted in 24 g ethanol /100 g dry biomass for dilute ammonia pretreated sorghum and 9 g ethanol /100 g dry biomass for untreated sorghum.
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