期刊
APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
卷 160, 期 7, 页码 1947-1954出版社
HUMANA PRESS INC
DOI: 10.1007/s12010-009-8755-0
关键词
Laccase covalent immobilization; Poly(vinyl alcohol) cryogel
资金
- Romanian Academy [1R-2008]
- Russian Foundation for Basic Research [07-03-91682_RA_a]
Laccases are enzymes belonging to the Oxidoreductases class. These enzymes may be good biocatalysts for different processes, at laboratory and industrial levels. A successful use at industrial scale demands a higher stability of the enzyme. As an easy way to obtain longer life biocatalysts, the immobilization process is recommended. Thus, the paper presents different ways of obtaining new biocatalysts by a laccase covalent immobilization on a macroporous carrier based on poly(vinyl alcohol) cryogel. Different procedures of covalent immobilization are described, the newly obtained biocatalysts being characterized. According to the experimental data, the stability of the immobilized enzyme increased and the pH profile changed, compared with those of the free enzyme.
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