期刊
MOLECULAR PLANT-MICROBE INTERACTIONS
卷 18, 期 7, 页码 652-658出版社
AMER PHYTOPATHOLOGICAL SOC
DOI: 10.1094/MPMI-18-0652
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Zur is a regulator of the high-affinity zinc uptake system in many bacteria. In Xanthomonas campestris pv. campestris 8004, a putative protein encoded by the open reading frame designated as XC1430 shows 42% amino acid similarity with the Zur of Eschetichia coli. An XC1430-disrupted mutant 1430nk was constructed by homologous suicide plasmid integration. 1430nk failed to grow in rich medium supplemented with Zn2+ at a concentration of 400 mu M and in nonrich medium supplemented with Zn2+ at a concentration of 110 M, whereas the wild-type strain grew well in the same conditions. In rich medium with 400 mu M Zn2+, 1430nk accumulated significantly more Zn2+ than the wild-type strain. 1430nk showed a reduction in virulence on the host plant Chinese radish (Raphanus sativus L. var. radiculus Pers.) and produced less extracellular polysaccharide (EPS) than did the wild-type strain in the absence of added zinc. These results revealed that XC1430 is a functional member of the Zur regulator family that controls zinc homeostasis, EPS production, and virulence in X. campestris pv. campestris.
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