期刊
JOURNAL OF LEUKOCYTE BIOLOGY
卷 78, 期 1, 页码 178-186出版社
WILEY
DOI: 10.1189/jlb.1004621
关键词
HSV; CD8(+) T cells; IgG; IgA
资金
- NIAID NIH HHS [R01 AI 4646 201] Funding Source: Medline
In a DNA immunization against Herpes simplex virus (HSV), we examined the ability of Plasniid-encoded interleukin-15 (pIL-15) to induce and maintain the mucosal B and T cell immune response. pIL-15 generate.d memory CD8(+) T cell responses that were threefold higher and mainly maintained in the spleen, but high levels of immunoglobulin A antibodies were induced and maintained long-term in the vaginal mucosa. Both of these enhanced components of the immune responses were recalled rapidly upon challenge with a lethal dose of HSV McKrae, affording protection in mice immunized with codelivery of pIL-15. Our results show for the first time that intranasal administration of pIL-15 along with plasmid-encoded glycoprotein B of HSV leads to enhancement of primary and memory CD8+ T cell responses as well as humoral immune response. Therefore, a mucosal immunization strategy that incorporates a potent cytokine such as IL-15, as an adjuvant might induce protective mucosal immune responses that constitute the initial barrier at mucosal portals of pathogen entry.
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