期刊
APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
卷 160, 期 2, 页码 386-392出版社
HUMANA PRESS INC
DOI: 10.1007/s12010-008-8320-2
关键词
Bacillus subtilis NX-2; Fermentation; Glycerol; Molecular weight; Poly(gamma-glutamic acid)
资金
- National Basic Research Program of China (973 Program) [2007CB714304]
- National High Technology Research and Development Program of China (863 Program) [2006AA03Z453]
- National Nature Science Foundation of China [20674038]
- Graduate Student Innovation Project of Jiangsu Province
Glycerol would stimulate the production of poly(gamma-glutamic acid) (gamma-PGA) and decrease its molecular weight in Bacillus subtilis NX-2. When 20 g/l glycerol was added in the medium, the yield of gamma-PGA increased from 26.7 +/- 1.0 to 31.7 +/- 1.3 g/l, and molecular weight of gamma-PGA decreased from 2.43 +/- 0.07 x 10(6) to 1.86 +/- 0.06 x 10(6) Da. In addition, it was found that the decrease of gamma-PGA chain length by glycerol would lead to the decrease of broth viscosity during the fermentation and enhanced the uptake of substrates, which could not only improve cell growth but also stimulate gamma-PGA production. Moreover, it was also found that glycerol could effectively regulate molecular weight between 2.43 +/- 0.07 x 10(6) and 1.42 +/- 0.05 x 10(6) Da with the concentration ranging from 0 to 60 g/l. This was the first time to discover such contribution of glycerol on gamma-PGA production in Bacillus genus. And the effects of glycerol on molecular weight of gamma-PGA would be developed to be an approach for the regulation of microbial gamma-PGA chain length, which is of practical importance for future commercial development of this polymer.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据