期刊
JOURNAL OF BACTERIOLOGY
卷 187, 期 13, 页码 4592-4597出版社
AMER SOC MICROBIOLOGY
DOI: 10.1128/JB.187.13.4592-4597.2005
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资金
- NIAID NIH HHS [1R21 AI05943] Funding Source: Medline
- PHS HHS [R01 B0055201] Funding Source: Medline
Certain carbohydrates (rhamnose, 3-O-methyl rhamnose, and galactosamine) have been demonstrated to be present in Bacillus anthracis spores but absent in vegetative cells. Others have demonstrated that these spore-specific sugars are constituents of the glycoprotein BclA. In the current work, spore extracts were separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. A second collagen-like glycoprotein, BclB, was identified in B. anthracis. The protein moiety of this glycoprotein was identified by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MS) and the carbohydrate components by gas chromatography-mass spectrometry and tandem mass spectrometry. Spore-specific sugars were also demonstrated to be components of BclB.
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