Mutations involving gains of glycosylation have been considered rare, and the pathogenic role of the new carbohydrate chains has never been formally established. We identified three children with mendelian susceptibility to mycobacterial disease who were homozygous with respect to a missense mutation in IFNGR2 creating a new N- glycosylation site in the IFN gamma R2 chain. The resulting additional carbohydrate moiety was both necessary and sufficient to abolish the cellular response to IFN gamma. We then searched the Human Gene Mutation Database for potential gain- of- N- glycosylation missense mutations; of 10,047 mutations in 577 genes encoding proteins trafficked through the secretory pathway, we identified 142 candidate mutations ( similar to 1.4%) in 77 genes ( similar to 13.3%). Six mutant proteins bore new N- linked carbohydrate moieties. Thus, an unexpectedly high proportion of mutations that cause human genetic disease might lead to the creation of new N- glycosylation sites. Their pathogenic effects may be a direct consequence of the addition of N- linked carbohydrate.
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