期刊
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
卷 102, 期 28, 页码 9796-9801出版社
NATL ACAD SCIENCES
DOI: 10.1073/pnas.0502917102
关键词
binding coefficient; multiple sites; transcription factors
资金
- NHGRI NIH HHS [HG 01506, R01 HG001506] Funding Source: Medline
We have performed single-molecule studies of GFP-Lacl repressor proteins bound to bacteriophage A DNA containing a 256 tandem lac operator insertion confined in nanochannels. An integrated photon molecular counting method was developed to determine the number of proteins bound to DNA. By using this method, we determined the saturated mean occupancy of the 256 tandem lac operators to be 13, which constitutes only 2.5% of the available sites. This low occupancy level suggests that the repressors influence each other even when they are widely separated, at distances on the order of 200 nm, or several DNA persistence lengths.
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