Spectroscopic and viscosity study of doxorubicin interaction with DNA

Photoirradiation of O-hydroxylcyclohexyletheramine-doxorubicin (dox) when bound to DNA exhibits high DNA cleavage efficiency and selectivity, leading to GG steps cleavage. Coupling ultraviolet absorption with viscosity measurement, the mode of dox-DNA complex is groove-binding. The three-dimensional structure from X-ray crystallography reveals that dox bound to DNA base pairs to groove reversal along with its antenna (hydroxylcyclohexylether-amine) in the major groove. We also provide a direct evidence of supporting a model that transfers electrons from base to dox and holes migrate within DNA. (c) 2005 Elsevier B.V. All rights reserved.