期刊
REGULATORY PEPTIDES
卷 129, 期 1-3, 页码 191-201出版社
ELSEVIER
DOI: 10.1016/j.regpep.2005.02.011
关键词
immunohistochemistry; central complex; alimentary canal; midgut endocrine cells; hindgut; ELISA; peptide
Crustacean cardioactive peptide (CLAP)-like immunoreactivity was localized and quantified in the central and peripheral nervous system of the Vietnamese stick insect, Baculum extradentatum, using immunohistochemistry and enzyme-linked immunosorbent assay (ELISA). The brain. frontal ganglion, suboesophageal ganglion and ventral nerve cord displayed neurons and processes with CLAP-like immunoreactivity. The brain, in comparison to the other parts of the central nervous system, contained the greatest amount of CCAP (167 +/- 18 fmol), and showed CCAP-like staining in neurons, neuropil regions and the central complex. There were also CLAP-like varicosities and processes associated with the corpus cardiacum. The alimentary canal of B. extradentatum contained CCAP with the largest amount localized in the midget (1110 +/- 274 fmol CLAP equivalents). The midgut contained numerous endocrine-like cells which stained positively for CCAP, whereas the foregut and hindgut revealed an extensive network of CCAP-like immunoreactive axons and varicosities. Based on physiological assays, the hindgut of the stick insect was found to be sensitive to CLAP, showing dose-dependent increases in contractions with threshold at 10(-10) M CLAP and maximal response at 5 x 10(-7) M CCAP. There were negligible quantities of CLAP in the oviducts and no CLAP-like immunoreactivity was associated with the oviducts. CLAP had no effect on spontaneous contractions of the oviducts. The presence of CCAP in the central nervous system, the stomatogastric nervous system, the corpus cardiacum and the alimentary canal, suggest broad ranging roles for CCAP in B. extradentatum. (c) 2005 Elsevier B.V. All rights reserved.
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