期刊
VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY
卷 106, 期 3-4, 页码 321-327出版社
ELSEVIER
DOI: 10.1016/j.vetimm.2005.03.003
关键词
chicken; cathelicidin; myeloid; innate immunity; uropygial gland
Cathelicidins, antimicrobial peptides with broad spectrum activity, have been almost exclusively found in mammals. Here, we report the cloning of a novel avian cathelicidin, chicken myeloid antimicrobial peptide 27 (CMAP27) from chicken bone marrow cells. A combined expressed sequence tag (EST) and genomic based search revealed a cathelicidin-like gene located at the terminus of chromosome 2. Reverse transcriptase-polymerase chain reaction (RT-PCR) and 5'RACE techniques resulted in a 154 amino acid prepropeptide, homologous to chicken cathelicidin 1 (51%) and most similar to a-helical myeloid antibacterial peptides (MAPs; 29-33%). A putative elastase cleavage site (LVQRG down arrow RF) suggests the production of a 27 amino acid antimicrobial peptide, predicted to adopt an a-helical configuration followed by a hydrophobic tail. Comparative analyses between antimicrobial peptide domains showed marked similarity between CMAP27 and MAP members of the bovidae family, but not with the alpha-helical chicken cathelicidin 1. Strongest expression of CMAP27 mRNA was found in myeloid/lymphoid tissues, testis and uropygial gland. In accordance with the phylogenetic tree analysis, these findings support the theory of a common ancestral cathelicidin gene and suggest an important role for cathelicidins in chicken innate host defense. (c) 2005 Elsevier B.V. All rights reserved.
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