期刊
JOURNAL OF PHYSIOLOGY-LONDON
卷 566, 期 2, 页码 505-518出版社
WILEY
DOI: 10.1113/jphysiol.2005.085985
关键词
-
资金
- NICHD NIH HHS [R03 HD045634, HD41002, R01 HD041002] Funding Source: Medline
- NINDS NIH HHS [R01 NS023941, NS23941, R56 NS023941] Funding Source: Medline
The firing pattern of oxytocin (OT) hormone synthesizing neurons changes dramatically immediately before each milk ejection, when a brief burst of action potentials is discharged. OT neurons possess intrinsic currents that would modulate this burst. Our previous studies showed the amplitude of the Ca2+-dependent afterhyperpolarization (AHP) following spike trains is significantly larger during lactation. In the present study we sought to determine which component of the AHP is enhanced, and whether the enhancement could be related to changes in whole-cell Ca2+ current or the Ca2+ transient in identified OT or vasopressin (VP) neurons during lactation. We confirmed, with whole-cell current-clamp recordings, our previous finding from sharp electrodes that the size of the AHP following spike trains increased in OT, but not VP neurons during lactation. We then determined that an apamin-sensitive medium-duration AHP (mAHP) and an apamin-insensitive slow AHP (sAHP) were specifically increased in OT neurons. Simultaneous Ca2+ imaging revealed that the peak change in somatic [Ca2+](i) was not altered in either cell type, but the slow decay of the Ca2+ transient was faster in both cell types during lactation. In voltage clamp, the whole-cell, Ca2+ current was slightly larger during lactation in OT cells only, but current density was unchanged when corrected for somatic hypertrophy. The currents, I-mAHp and I-sAHp, also were increased in OT neurons only, but only the apamin-sensitive I-mAHp showed an increase in current density after adjusting for somatic hypertrophy. These findings suggest a specific modulation (e.g. increased number) of the small-conductance Ca2+-dependent K+ (SK) channels, or their interaction with Ca2+, underlies the increased mAHP/I-mAHp during lactation. This larger mAHP may be necessary to limit the explosive bursts during milk ejection.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据